Abstract Thrips are major agricultural pests globally and spinosyn insecticides like spinosad and spinetoram are commonly used for their control. However, numerous cases of resistance have emerged, often linked to mutations in the nicotinic acetylcholine receptor (nAChR) α6 subunit, the main molecular target of spinosyns. In this study, toxicological data for spinosad and spinetoram were obtained from a susceptible strain of Frankliniella occidentalis , as well as two fiel

Abstract Parasitoid wasps contribute to biological control services. Parasitoid adults are highly dependent on sugar-rich resources. Adding flowering plants providing nectar in crop fields promotes parasitoid wasps, but not always pest parasitism because addition of flowering plants may attract parasitoid species that are not directly involved in the control of crop pests. In a factorial experiment conducted for one year in pesticide-free orchards, we analysed the effects of

Abstract The green peach aphid, Myzus persicae , is an economically important crop pest that has evolved resistance to numerous insecticides used for control. Recent research has shown that populations of M. persicae in Greece have evolved resistance to flupyradifurone, a butenolide insecticide that acts as a reversible agonist on insect nicotinic acetylcholine receptors (nAChRs). However, the genetic mechanisms underpinning resistance remain unclear. Here we used transcript

Abstract Pesticide resistance in agricultural pests poses a significant challenge to sustainable crop protection. In this study, we assessed the current resistance status of two key pests, Tetranychus urticae and Bemisia tabaci, in major agricultural regions of Greece. A total of 25 field populations were collected between 2023 and 2025 and tested using single-dose toxicity bioassays and droplet digital PCR (ddPCR) for known resistance-associated target-site mutations. The re

Abstract Spider mites and thrips are major agricultural pests worldwide. Despite their well-documented impact on crop yields in South-East Asia, their species composition, distribution, and pesticide susceptibility remain poorly studied in the region. This study focused on the Red River Delta region of Vietnam. Multiple populations of spider mites and thrips were collected and subjected to phylogenetic analysis for reliable species identification. For the first time, Tetranyc

Abstract Spider mites are significant agricultural pests in apple orchards, where their control relies heavily on acaricide applications. In this study, seven populations of Tetranychus turkestani were collected from apple orchards in Isfahan Province, Iran, to evaluate their susceptibility to hexythiazox and etoxazole. Probit analysis revealed significant resistance to hexythiazox in T. turkestani populations, with resistance ratios (RRs) ranging from 290- to 2550-fold, w

Abstract The CncC/Keap1 signalling pathway regulates antioxidant and detoxification gene expression in arthropods and is frequently associated with metabolic insecticide resistance. This review critically assesses evidence for its role in resistance phenotypes across key pest species. Although overactivation of CncC/Keap1 correlates with increased detoxification enzyme expression and pesticide tolerance, causal mutations in the coding or regulatory regions of CncC , Keap1 , o

Abstract The cotton mealybug Phenacoccus solenopsis (Hemiptera: Pseudococcidae) is a polyphagous invasive species native to America and...

Abstract Echinothrips americanus Morgan (Thysanoptera: Thripidae), also known as poinsettia thrips, has invaded several parts of the...